You are using IE 8 or below so some features may not work. Please upgrade to a modern browser
Ihr Webbrowser ist veraltet und wird leider nicht in vollem Umfang unterstützt.

Analytical Methods

A wide range of analytical methods is available for testing of intermediates, APIs and finished drug products. They can be readily adapted for new products. Qualification and validation of analytical methods for each project are performed according ICH guidelines.
A list of the main methodological groups is given below. Additional methods are organised with qualified contract analytical laboratories.


  • size / size distribution (polydispersity index)
  • zeta potential


  • sterility
  • germ count


qualitative and semi-quantitative using a wide range of detector systems, e.g. fluorescence, chemiluminescence

  • sodium dodecylsulfate polyacrylamid gel electrophoresis (SDS-PAGE)
  • isoelectric focusing (IEF)
  • Western blot

Microtiter Assays

  • enzyme linked immunosorbent assay (ELISA)
  • protein activity assay
  • endotoxin
  • enzyme activity assay

Instrumental Analytics

all conventional separation methods in combination with various detector systems

  • high performance liquid chromatography (HPLC)
  • size exclusion chromatography-HPLC (SEC-HPLC)
  • reversed phase-HPLC (RP-HPLC)
  • capillary zone electrophoresis (CZE)
  • optical density (OD)
  • fourier transform infrared spectroscopy (FTIR)
  • fluorescence spectroscopy
  • photon correlation spectroscopy
  • zeta potential

In Vitro Test Systems

  • proliferation assays, e.g. using MTT, XTT, BrdU
  • toxicity assays


  • osmolality
  • conductivity
  • pH
  • density
  • residual moisture (Karl Fischer)
  • visual appearance
  • TOC

Analytical Methods


5th International mRNA Health Conference
November 1-2, 2017

Berlin, Germany

Dr. Andreas Wagner
Dr. Charalampos Koutsoulas

November 7-10, 2017

Vienna, Austria

Dr. Andreas Wagner
Dr. Charalampos Koutsoulas
Harald Loidl, BSc
Frances Aichinger, BSc



Polymun Liposome Technology 
Broschure (pdf, 1MB)