Downstream Processing

Polymun has gathered experience in designing purification processes for proteins of all kinds, for antibodies, hormones and enzymes. GMP-compliance and industrial applicability are central to all operations.

Polymun uses a variety of methods, combining them for each protein for the optimal solution. Next to yield and purity with depletion of endotoxins, host cell protein and DNA or adventitious viruses, the focus is given on validation and scalability. The downstream purification capacity matches the fermentation output of our GMP-plant with process systems up to 600 L/h and 6 bar.

Cell Disintegration

high pressure homogenization

Separation

centrifugation
depth filtration
sterile filtration
nano filtration
ultra/dia filtration

Chromatography

in packed bed as well as expanded bed adsorption mode
ion exchange (IEX)
affinity (AC) e.g. protein A, protein G, specific antibody, specific ligand etc.
size exclusion (SEC)
hydrophobic interaction (HIC)
reversed phase (RPC)
mixed-mode (MMC)

Precipitation

ammonium sulfate