Analytical Tools
A wide range of analytical methods is available that can be readily adapted to new requirements. A list of the main methodological groups is given below. Furthermore, new test systems can be set up, e.g. an ELISA for a new protein or an in vitro test for specific biologic activity.
Microbiology
sterilitygerm count
air and water germ count
plasmid stability
Electrophoresis
qualitative and semi-quantitative using a wide range of detector systems, e.g. fluorescence, chemiluminescencesodium dodecylsulfate polyacrylamid gel electrophoresis (SDS-PAGE)
isoelectric focusing (IEF)
Western blot
2D electrophoresis
fluorescence 2D difference gel electrophoresis (2D DIGE)
capillary electrophoresis
Microtiter Assays
enzyme linked immunosorbent assay (ELISA)protein activity assay
host cell protein
protein A
endotoxin
enzyme activity assay
Instrumental Analytics
all conventional separation methods in combination with various detector systemshigh performance liquid chromatography (HPLC)
size exclusion chromatography-HPLC (SEC-HPLC)
reversed phase-HPLC (RP-HPLC)
capillary zone electrophoresis (CZE)
optical density (OD)
DNA threshold method
fluorescence spectroscopy
photon correlation spectroscopy
zeta potential
Cell Culture Analytics
mycoplasm (by DNA-staining)In Vitro Test Systems
proliferation assays, e.g. using MTT, XTT, BrdUtoxicity assays
Microscopy
fluorescence microscopyphase contrast microscopy
confocal microscopy