Analytical Tools

A wide range of analytical methods is available that can be readily adapted to new requirements. A list of the main methodological groups is given below. Furthermore, new test systems can be set up, e.g. an ELISA for a new protein or an in vitro test for specific biologic activity.

Microbiology

sterility
germ count
air and water germ count
plasmid stability

Electrophoresis

qualitative and semi-quantitative using a wide range of detector systems, e.g. fluorescence, chemiluminescence
sodium dodecylsulfate polyacrylamid gel electrophoresis (SDS-PAGE)
isoelectric focusing (IEF)
Western blot
2D electrophoresis
fluorescence 2D difference gel electrophoresis (2D DIGE)
capillary electrophoresis

Microtiter Assays

enzyme linked immunosorbent assay (ELISA)
protein activity assay
host cell protein
protein A
endotoxin
enzyme activity assay

Instrumental Analytics

all conventional separation methods in combination with various detector systems
high performance liquid chromatography (HPLC)
size exclusion chromatography-HPLC (SEC-HPLC)
reversed phase-HPLC (RP-HPLC)
capillary zone electrophoresis (CZE)
optical density (OD)
DNA threshold method
fluorescence spectroscopy
photon correlation spectroscopy
zeta potential

Cell Culture Analytics

mycoplasm (by DNA-staining)

In Vitro Test Systems

proliferation assays, e.g. using MTT, XTT, BrdU
toxicity assays

Microscopy

fluorescence microscopy
phase contrast microscopy
confocal microscopy